Cavity Ring Down Spectroscopy as a calibration tool for absolute fluorescence measurements

Metrology in Life Sciences

Recently developed microstructured electrode arrays (MSE) generate planar plasma disĀ­charges at high pressures (up to ambient pressure) where only moderate voltages (<300V) are applied. These devices are further developed to generate plasma at atmospheric pressures and use it for the removal of pollutants, chemical synthesis, methane activation, and to functionalize surfaces on a micrometer scale for the use as bio assays. For the analysis of the plasma and molecular characteristics different spectroscopic techniques are used, like emission spectroscopy, FTIR spectroscopy, laser induces fluorescence (LIF), and Cavity-Ring-Down (CRD) spectroscopy. A specific coupling between the (sensitive, but relative) fluorescence techniques with the absolute CRD technique will allow for the first time an absolute analysis of the involved species with high precision. This allows elucidating the involved mechanisms and to understand and optimize the plasma process. The employed apparatus consists of laser systems (Nd:Yag and excimer pumped dye laser and a femto second laser system), an ICCD camera detection system, fluorescence spectrometer, a time-of-flight mass spectrometer, and high frequency detection devices. The research work is performed with other research groups from Institutes of the University and the Fraunhofer Institute.

TU Braunschweig
Ph.D. student: